Journal: Oncology Letters
Article Title: Analysis of the clonal origin and differences in the biological behavior of multifocal papillary thyroid carcinoma
doi: 10.3892/ol.2024.14677
Figure Lengend Snippet: BRAF V600E mutation in multifocal papillary thyroid carcinoma. Representative (A) BRAF V600E mutant and (B) wild-type amplification plots generated using quantitative PCR. The red marking represents the threshold line. BRAF, v-raf murine sarcoma viral oncogene homolog B1; BRAF-IC, BRAF internal reference gene detection curve; BRAF-W, BRAF V600E wild-type gene detection curve; BRAF-M, BRAF V600E mutation gene detection curve; DRn, change in relative fluorescence normalized.
Article Snippet: The primary antibodies used were mouse anti-human BRAF monoclonal antibody (cat no. TA500431) and rabbit anti-human TERT polyclonal antibody (cat. no. TA324097), both from OriGene Technologies, Inc, a dilution of 1:200.
Techniques: Mutagenesis, Amplification, Generated, Real-time Polymerase Chain Reaction, Fluorescence